Overview
A position is available for a motivated postdoctoral research scientist (commencing in early 2011) with an interest in gene transcription and chromatin biology. The project will be led by Dr Alastair Fleming at Trinity College Dublin, Ireland, and is funded by a Wellcome Trust Project Grant award to Dr Fleming.
Salary Range: Postdoctoral Research Fellow: €40,000 per annum
Project Outline
This exciting project will address the fundamental question of how eukaryotic transcription occurs in the context of chromatin using Saccharomyces cerevisiae as a model organism. The basic subunit of chromatin, the nucleosome, comprises DNA wrapped around an octamer of histone proteins. During transcription elongation, nucleosomes are evicted in front of RNA polymerase and reassembled in its wake. Nucleosome eviction allows polymerase passage, and reassembly acts to maintain chromatin structure and prevent cryptic transcription initiation from occurring within transcribed genes.
In this project you will examine the contribution of histone H2B monoubiquitylation and histone chaperones towards the regulation of the nucleosome dynamics required to promote efficient transcription elongation.
The interplay between RNA polymerase II, nucleosomes and histone chaperones will be directly analyzed during transcription using a gene-specific in-vivo kinetic ChIP assay. Next generation ChIP-Seq and RNA-Seq approaches will determine the global landscape of chromatin structure across actively transcribed genes. Yeast genetics will underpin this study.
The successful candidate will join the Fleming laboratory at Trinity College Dublin, which focuses on chromatin structure and function in the yeast, Saccharomyces cerevisiae. For more details see http://www.tcd.ie/Microbiology/research/a_fleming.php and Fleming et al., 2008, Molecular Cell, 31, pg 57-66.
Requirements for the Postdoctoral Fellowship: Applicants should have recently completed, or be close to completion, of a relevant Ph.D. Molecular microbiological expertise will be important, and experience with gene regulation, chromatin biology and functional genomics or next-generation sequencing would be ideal.
Further details: To learn more about the School of Genetics and Microbiology, please visit http://www.genetics-microbiology.tcd.ie/
Dublin: Dublin universities have an impressive track record in microbial gene regulation biology.
Closing Date: 12 Noon on Friday 10th December 2010
Interested candidates should contact Dr Alastair Fleming (alastair.fleming@tcd.ie; Tel: +353 1896 3112) for an informal discussion.
Application procedure:
Candidates are invited to send PDF copies of a full CV (detailing their qualifications and experience, to include the names and contact details of 3 referees, with email addresses), together with a covering letter to:
Christine Devlin
Recruitment Executive
Staff Office
House No. 4
Trinity College
Dublin 2
Ireland
Tel: +353 1 896 3584
Fax: +353 1 677 2694
For applications via email: christine.devlin@tcd.ie
Please kindly mention Scholarization.blogspot.com when applying for this scholarship
A position is available for a motivated postdoctoral research scientist (commencing in early 2011) with an interest in gene transcription and chromatin biology. The project will be led by Dr Alastair Fleming at Trinity College Dublin, Ireland, and is funded by a Wellcome Trust Project Grant award to Dr Fleming.
Salary Range: Postdoctoral Research Fellow: €40,000 per annum
Project Outline
This exciting project will address the fundamental question of how eukaryotic transcription occurs in the context of chromatin using Saccharomyces cerevisiae as a model organism. The basic subunit of chromatin, the nucleosome, comprises DNA wrapped around an octamer of histone proteins. During transcription elongation, nucleosomes are evicted in front of RNA polymerase and reassembled in its wake. Nucleosome eviction allows polymerase passage, and reassembly acts to maintain chromatin structure and prevent cryptic transcription initiation from occurring within transcribed genes.
In this project you will examine the contribution of histone H2B monoubiquitylation and histone chaperones towards the regulation of the nucleosome dynamics required to promote efficient transcription elongation.
The interplay between RNA polymerase II, nucleosomes and histone chaperones will be directly analyzed during transcription using a gene-specific in-vivo kinetic ChIP assay. Next generation ChIP-Seq and RNA-Seq approaches will determine the global landscape of chromatin structure across actively transcribed genes. Yeast genetics will underpin this study.
The successful candidate will join the Fleming laboratory at Trinity College Dublin, which focuses on chromatin structure and function in the yeast, Saccharomyces cerevisiae. For more details see http://www.tcd.ie/Microbiology/research/a_fleming.php and Fleming et al., 2008, Molecular Cell, 31, pg 57-66.
Requirements for the Postdoctoral Fellowship: Applicants should have recently completed, or be close to completion, of a relevant Ph.D. Molecular microbiological expertise will be important, and experience with gene regulation, chromatin biology and functional genomics or next-generation sequencing would be ideal.
Further details: To learn more about the School of Genetics and Microbiology, please visit http://www.genetics-microbiology.tcd.ie/
Dublin: Dublin universities have an impressive track record in microbial gene regulation biology.
Closing Date: 12 Noon on Friday 10th December 2010
Interested candidates should contact Dr Alastair Fleming (alastair.fleming@tcd.ie; Tel: +353 1896 3112) for an informal discussion.
Application procedure:
Candidates are invited to send PDF copies of a full CV (detailing their qualifications and experience, to include the names and contact details of 3 referees, with email addresses), together with a covering letter to:
Christine Devlin
Recruitment Executive
Staff Office
House No. 4
Trinity College
Dublin 2
Ireland
Tel: +353 1 896 3584
Fax: +353 1 677 2694
For applications via email: christine.devlin@tcd.ie
Please kindly mention Scholarization.blogspot.com when applying for this scholarship
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