Overview
As a post-doctoral researcher you will comprehensively characterize ERα induced gene regulation in MCF7 (breast cancer) and MCF10 cells (“normal” breast epithelial). You will generate and describe transcriptional networks for ERα, NR-interacting factors such as p65 using ChIP-seq to define dynamic histone, DNA methylation and chromatin marks in conjunction with RNA-seq to report the transcriptome.
The obtained unique and comprehensive data set on ERα-induced gene regulation will be complemented by positional definition of the association of distal enhancer elements with NR regulated promoters using genome wide long-range interaction approaches. In collaboration with SYNERGY partners, computational methods, combined with biomedical knowledge and leading technology to describe genome-wide gene regulation at a molecular and mechanistic level, will be used to describe and predict gene networks regulated by ERα.
Requirements
You have a PhD in Molecular Biology, Biochemistry, Genomics or related field, strong analytical skills and are motivated to conduct scientific research. You are very meticulous and a good team player. Experience with generation and bioinformatic analysis of large data sets is a plus, but is not required.
Organization Background
Research at the department of Molecular Biology (NCMLS) aims at unravelling the regulatory networks of transcription factors, epigenetic modifications and chromatin remodelling factors that specify development and differentiation in the context of health and disease. The department is at the forefront of (epi)genetic research using genome wide approaches to shed light on pathways and mechanisms in transcription regulation. The department participates in several multinational consortia, including SYNERGY which aims to gain a comprehensive, quantitative and predictive understanding of oestrogen receptor alpha (ERα) regulated gene regulatory networks in breast cancer cells by applying state-of-the-art experimental technologies with cutting- edge bioinformatics and mathematical modelling performed in close collaboration with SYNERGY partners.
Website: http://www.umcn.nl
Conditions of employment
Employment: 1,0 fte
Maximum salary per month, based on a fulltime employment: € 3,755 gross/month
Salary starts at 10.4 on the wage scale, depending on previous work experience.
Salary scale: 10
Additional conditions of employment
Initially 1 year, with the possibility of extension to the full duration of the EraBioSys grant (3 years).
Additional Information
Prof. Henk Stunnenberg
Telephone: +31 24 3610524
E-mail: h.stunnenberg@ncmls.ru.nl
Application
You can apply for the job (mention the vacancy number 62.02.10) before 1 February 2010 by sending your application -preferably by email- to:
Radboud University Nijmegen / NCMLS
Prof. Henk Stunnenberg
Department of Molecular Biology,
#274
Geert Grooteplein 28, 6525 GA NIJMEGEN, NL
Telephone: +31 24 3610524
E-mail: h.stunnenberg@ncmls.ru.nl
Please kindly mention Scholarization.blogspot.com when applying for this opportunity
As a post-doctoral researcher you will comprehensively characterize ERα induced gene regulation in MCF7 (breast cancer) and MCF10 cells (“normal” breast epithelial). You will generate and describe transcriptional networks for ERα, NR-interacting factors such as p65 using ChIP-seq to define dynamic histone, DNA methylation and chromatin marks in conjunction with RNA-seq to report the transcriptome.
The obtained unique and comprehensive data set on ERα-induced gene regulation will be complemented by positional definition of the association of distal enhancer elements with NR regulated promoters using genome wide long-range interaction approaches. In collaboration with SYNERGY partners, computational methods, combined with biomedical knowledge and leading technology to describe genome-wide gene regulation at a molecular and mechanistic level, will be used to describe and predict gene networks regulated by ERα.
Requirements
You have a PhD in Molecular Biology, Biochemistry, Genomics or related field, strong analytical skills and are motivated to conduct scientific research. You are very meticulous and a good team player. Experience with generation and bioinformatic analysis of large data sets is a plus, but is not required.
Organization Background
Research at the department of Molecular Biology (NCMLS) aims at unravelling the regulatory networks of transcription factors, epigenetic modifications and chromatin remodelling factors that specify development and differentiation in the context of health and disease. The department is at the forefront of (epi)genetic research using genome wide approaches to shed light on pathways and mechanisms in transcription regulation. The department participates in several multinational consortia, including SYNERGY which aims to gain a comprehensive, quantitative and predictive understanding of oestrogen receptor alpha (ERα) regulated gene regulatory networks in breast cancer cells by applying state-of-the-art experimental technologies with cutting- edge bioinformatics and mathematical modelling performed in close collaboration with SYNERGY partners.
Website: http://www.umcn.nl
Conditions of employment
Employment: 1,0 fte
Maximum salary per month, based on a fulltime employment: € 3,755 gross/month
Salary starts at 10.4 on the wage scale, depending on previous work experience.
Salary scale: 10
Additional conditions of employment
Initially 1 year, with the possibility of extension to the full duration of the EraBioSys grant (3 years).
Additional Information
Prof. Henk Stunnenberg
Telephone: +31 24 3610524
E-mail: h.stunnenberg@ncmls.ru.nl
Application
You can apply for the job (mention the vacancy number 62.02.10) before 1 February 2010 by sending your application -preferably by email- to:
Radboud University Nijmegen / NCMLS
Prof. Henk Stunnenberg
Department of Molecular Biology,
#274
Geert Grooteplein 28, 6525 GA NIJMEGEN, NL
Telephone: +31 24 3610524
E-mail: h.stunnenberg@ncmls.ru.nl
Please kindly mention Scholarization.blogspot.com when applying for this opportunity
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