Overview
In our investigation of Tricholoma vaccinum-spruce interaction, we identified, among other genes induced in the mutual symbiosis, an aldehyde dehydrogenase gene. This gene is induced by ethanol, but also by indole acete aldehyde, and heterologous expression could show conversion of IAAld by the encoded enzyme. We are currently deducing the correct synthesis pathway for IAA in this agaricomycete. At the same time as producing the phytohormone, the fungus in pure culture responds by enhanced ramification, a feature needed for establishing the Hartig’ net in symbiosis. Within the completed genome sequences of the related fungus Schizophyllum commune, as well as of the ectomycorrhizal genus Laccaria bicolor, neither auxin efflux nor influx homologues could be detected. Thus, the screening for response induced genes will be vital to identify components of this interaction. The induction of IAA production could be shown by feeding the precursor tryptophan, but the same GC-MS changes were observed if a signaling compound is applied. For functional analysis, we established a transformation system for T. vaccinum, allowing us to over-express genes. Response to this inducing compound will be investigated by differential display.
International Max Planck Research School:
“The Exploration of Ecological Interactions with Molecular and Chemical Techniques”
The International Max Planck Research School (IMPRS) "The Exploration of Ecological Interactions with Molecular and Chemical Techniques" in Jena, Germany, is offering 6 PhD fellowships. Students holding a Master’s degree (or equivalent) in molecular biology, ecology, plant physiology, entomology, microbiology, chemistry, biochemistry, neuroethology or bioinformatics, with a proven record of success in one of the disciplines and interest in examining ecological interactions, are eligible to conduct a doctoral project within the IMPRS.
The Research School is a joint initiative of the Max Planck Institute for Chemical Ecology, Friedrich Schiller University, Jena, the Leibniz Institute for Natural Product Research and Infection Biology, and the Leibniz Institute of Plant Genetics and Crop Plant Research. The main focus is on plant-herbivore interactions; other ecologically relevant topics are also investigated.
Application deadline is June 30, 2010. For detailed information about the IMPRS, PhD topics offered and application requirements, please visit our website http://imprs.ice.mpg.de/.
Ectomycorrhizal Signaling
Prof. Erika Kothe, FSU, Prof. Wilhelm Boland, MPI-CE
How to apply:
Apply online at
https://imprs-reg.ice.mpg.de/rs/reg/register.php
Please kindly mention Scholarization.blogspot.com when applying for this opportunity
In our investigation of Tricholoma vaccinum-spruce interaction, we identified, among other genes induced in the mutual symbiosis, an aldehyde dehydrogenase gene. This gene is induced by ethanol, but also by indole acete aldehyde, and heterologous expression could show conversion of IAAld by the encoded enzyme. We are currently deducing the correct synthesis pathway for IAA in this agaricomycete. At the same time as producing the phytohormone, the fungus in pure culture responds by enhanced ramification, a feature needed for establishing the Hartig’ net in symbiosis. Within the completed genome sequences of the related fungus Schizophyllum commune, as well as of the ectomycorrhizal genus Laccaria bicolor, neither auxin efflux nor influx homologues could be detected. Thus, the screening for response induced genes will be vital to identify components of this interaction. The induction of IAA production could be shown by feeding the precursor tryptophan, but the same GC-MS changes were observed if a signaling compound is applied. For functional analysis, we established a transformation system for T. vaccinum, allowing us to over-express genes. Response to this inducing compound will be investigated by differential display.
International Max Planck Research School:
“The Exploration of Ecological Interactions with Molecular and Chemical Techniques”
The International Max Planck Research School (IMPRS) "The Exploration of Ecological Interactions with Molecular and Chemical Techniques" in Jena, Germany, is offering 6 PhD fellowships. Students holding a Master’s degree (or equivalent) in molecular biology, ecology, plant physiology, entomology, microbiology, chemistry, biochemistry, neuroethology or bioinformatics, with a proven record of success in one of the disciplines and interest in examining ecological interactions, are eligible to conduct a doctoral project within the IMPRS.
The Research School is a joint initiative of the Max Planck Institute for Chemical Ecology, Friedrich Schiller University, Jena, the Leibniz Institute for Natural Product Research and Infection Biology, and the Leibniz Institute of Plant Genetics and Crop Plant Research. The main focus is on plant-herbivore interactions; other ecologically relevant topics are also investigated.
Application deadline is June 30, 2010. For detailed information about the IMPRS, PhD topics offered and application requirements, please visit our website http://imprs.ice.mpg.de/.
Ectomycorrhizal Signaling
Prof. Erika Kothe, FSU, Prof. Wilhelm Boland, MPI-CE
How to apply:
Apply online at
https://imprs-reg.ice.mpg.de/rs/reg/register.php
Please kindly mention Scholarization.blogspot.com when applying for this opportunity
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